Биотин-PEG4-N3,

Описание

Biotin-PEG₃-azide is a PEGylated version of biotin-azide that is more hydrophilic due to the incorporation of a PEGylated spacer. It can be used to label terminal alkynes via copper-catalyzed click reactions or copper-free click reactions with cyclooctynes., Biotin-PEG₃-azide has been used to non-enzymatically and covalently tag proteins, which can be separated and detected using common laboratory methods such as SDS-PAGE and Western blot. It can be used in combination with other protein tags. Biotin-PEG₃-azide has also been used in nanostructured biointerfaces to study ligand and receptor spacing when there are different signaling molecules present.

Использование

Biotinylation reagent for labeling alkyne containing molecules or biomolecules using either copper-catalyzed 1,3 dipolar cycloaddition click chemistry or copper-free click chemistry with cyclooctyne derivatives. The azide group reacts with alkynes to form a stable triazole linkage facilitating the introduction of biotin into your alkyne modified system of interest. This reaction is bioorthogonal or in other words compatible with biological systems in that it?s components do not react with the biological environment.