ЕС 3.4.22.1

Использование

Cathepsin B from human liver has been used:

• as a positive control in the screening Cathepsin B inhibitor assay
• in wall extension (expansin activity) assay
• in microinjection human foreskin fibroblasts to test its effect on apoptosis induction

Общее описание

Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.

Методы очистки

Cathepsin B is purified by affinity chromatography on the semicarbazone of Gly-Phe-glycinal-linked to Sepharose 4B, with elution by 2,2'-dipyridyl disulfide [Rich et al. Biochem J 235 731 1986, Methods Enzymol 80 551 1981].