NDUFA10抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Mouse heart tissue, Mouse kidney tissue, Primary antibody: P01574(NDUFA10 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds    

  
  

NDUFA10 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10) is a critical component of mitochondrial Complex I, the largest enzyme in the electron transport chain (ETC) responsible for oxidizing NADH and transferring electrons to ubiquinone during ATP production. As a nuclear-encoded subunit, NDUFA10 localizes to the mitochondrial inner membrane and plays a structural and functional role in stabilizing Complex I and regulating its enzymatic activity. Dysregulation or mutations in NDUFA10 have been linked to mitochondrial disorders, neurodegenerative diseases (e.g., Parkinson’s and Alzheimer’s), and cancer, as impaired Complex I function disrupts cellular energy metabolism and redox balance.

Antibodies targeting NDUFA10 are essential tools for studying its expression, localization, and interactions within Complex I. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein levels in disease models, genetic knockouts, or under metabolic stress. These antibodies help elucidate mechanisms underlying mitochondrial dysfunction, such as oxidative phosphorylation deficits or reactive oxygen species (ROS) overproduction. Commercial NDUFA10 antibodies are typically raised against specific epitopes (e.g., human NDUFA10 peptides) and validated for cross-reactivity in common model organisms. Researchers also employ them to investigate therapeutic interventions targeting mitochondrial metabolism or to diagnose Complex I deficiencies in clinical samples. Proper validation, including knockout controls, is crucial to ensure specificity due to the high homology among mitochondrial proteins.