3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.
3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride has been used:
- in the detection of hydrogen peroxide in rice leaves
- as a substrate for rabbit peroxidase in immunohistochemistry of mice brain sections
- in immunocytochemistry of muscle-derived stem cells (MDSCs)
A substrate for peroxidase, and a reagent for the spectrophotometric determination of selenium
3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase.
3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase. This feature is exploited for visualization in histochemical (HC) and immunohistochemical (IHC) staining.
Suspected carcinogen withexperimental neoplastigenic and tumorigenic data. Poisonby intraperitoneal route. When heated to decomposition itemits very toxic fumes of HCl and NOx.
Dissolve the salt in water and precipitate it by adding conc HCl, then drying it over solid NaOH. [Beilstein 13 IV 530.]
