Phosphoenolpyruvate carboxykinase (PEPCK) is a hormonally regulated enzyme responsible for the first committed step in gluconeogenesis, catalyzing the conversion of oxaloacetate to phosphoenolpyruvate.1,2 PEPCK mRNA is most abundant in the liver, kidney cortex, and white adipose tissue but is also found in brown fat, the mammary gland during lactation, and the small intestine.2 Both non-insulin-dependent diabetes mellitus and streptozotocin-induced diabetes result in elevated PEPCK activity, protein, and mRNA.1,3 Two isozymes of PEPCK have been identified, cytosolic and mitochondrial.4 Cayman’s PEPCK polyclonal antibody was generated against murine cytosolic PEPCK and detects the protein at 63 kDa by western blotting in rat and murine samples.WARNING This product is not for human or veterinary use.
