Ub-Prg (also called Ub-PA) can react with the active Cys of DUBs through 1,2-addition
to produce a stable vinyl thioether product. To explain regulatory mechanisms, Ub-Prg
was used to capture the catalytic conformation by which RPN13DEUBAD activates
UCH-L5; and the catalytic conformation that truncated INO80DEUBAD lacks to inhibit
UCH-L5.
