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Xylene Cyanole FF

Product NameXylene Cyanole FF
CAS2650-17-1
MFC25H28N2O6S2.Na
MW539.62
EINECS220-167-5
MOL File2650-17-1.mol

Chemical Properties

Melting point	295°C
storage temp.	room temp
solubility	H2O: complete1mg/mL, clear, blue
form	Solid
Colour Index	42135
color	Green
PH Range	2.9 (purple) - 4.6 (green)(Methyl Orange - Xylene Cyanol)
Water Solubility	Soluble in water.
λmax	615 nm
Stability	Stable. Moisture-sensitive. Incompatible with strong oxidizing agents.
Major Application	diagnostic assay manufacturing hematology histology
InChIKey	JFQQXRTVXHQUCR-UHFFFAOYSA-N
SMILES	C(=C1C=CC(C(=C1)C)=NCC)(C1C=CC(=C(C=1)C)NCC)C1C=CC(=CC=1S(O)(=O)=O)S(O)(=O)=O.[Na]
CAS DataBase Reference	2650-17-1(CAS DataBase Reference)
EPA Substance Registry System	1,3-Benzenedisulfonic acid, 4-[[4-(ethylamino)-3-methylphenyl][4-(ethylimino)-3-methyl-2,5-cyclohexadien-1-ylidene]methyl]-, monosodium salt (2650-17-1)

Safety Information

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36-24/25
WGK Germany	3
TSCA	TSCA listed
HS Code	32041900
Storage Class	11 - Combustible Solids
Hazard Classifications	Eye Irrit. 2

MSDS

Provider	Language
Sodium hydrogen 4-[[4-(ethylamino)-m-tolyl][4-(ethylimino)-3-methylcyclohexa-2,5-dien-1-ylidene]methyl]benzene-1,3-disulphonate	English

Usage And Synthesis

Chemical Properties

dark green or blue-grey crystals

Uses

Xylene Cyanol FF is a tracking dye commonly used for nucleic acids. The compound has been used in both electrophoresis polyacrylamide and agarose gels for various evaluations including DNA sequencing. The compound has also been used in a mobility shift analysis of ssDNA. In addition, two highly sensitive spectrophotometric assays have been developed for the detection of platinum (IV) (sc-250738, Platinum(IV) chloride) and Chromium (sc-214714) with the use of Xylene Cyanol FF. As an organic dye-inactivated Sendai virus vaccine, Xylene Cyanol FF displayed strong protection against the virus.

Uses

Suitable as a tracking dye for DNA sequencing.

Preparation

4-Formylbenzene-1,3-disulfonic acid (1 Moore) and N-ethyl-2-methylbenzenamine (2 Moore) condensation, garnish with heavy chromate salt-oxalic acid oxidation its product.

General Description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The tracking dye typically migrates with the DNA molecules around 5kb. Xylene cyanol may also be used in loading buffer that facilitates the observation of the sample during gel loading and electrophoresis.

Biochem/physiol Actions

Xylene Cyanol FF acts as nucleic acid stain.

Properties and Applications

bright blue. Soluble in water to blue.

Standard	Light Fastness	Soaping		Persperation Fastness	Oxygen bleaching		Fastness to seawater
Standard	Light Fastness	Fading	Stain	Persperation Fastness	Fading	Stain	Fading	Stain
ISO	2	2	3-4	2-3	1		2-3	1
AATCC	2	4-5	4-5	2-3	1		2	2

Xylene Cyanole FF

Chemical Properties

Safety Information

MSDS

Usage And Synthesis

Preparation Products And Raw materials

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