L-去甲肾上腺素酒石酸氢盐(酯)
L-去甲肾上腺素酒石酸氢盐(酯) 性质
| 储存条件 | Store at 4°C, protect from light |
|---|---|
| 溶解度 | DMSO 中 31.93mg/mL |
| 酸度系数(pKa) | 8.64(at 25℃) |
| 熔点 | 102-104 °C |
| 水溶解性 | 31.93mg/mL in Water |
| CAS 数据库 | 51-40-1(CAS DataBase Reference) |
L-去甲肾上腺素酒石酸氢盐(酯) 用途与合成方法
EC50: 5.37 μM (β 1 -selective adrenergic receptor).
Norepinephrine (NE) bitartrate monohydrate is generally considered to be a β 1 -subtype selective adrenergic agonist. Norepinephrine (NE) also has direct activity at the β 2 -adrenoceptor in higher concentrations. Adipocytes from the inguinal fat pad (iWA) or the interscapular fat pad (BA) are isolated from neonatal wild-type C57BL/6J mice and cultured. To examine the effect of activating AT2 upon β-adrenergic signaling, cAMP production is first assessed in response to Norepinephrine (NE, 10 µM) with or without CGP (10 nM) co-treatment. Norepinephrine (NE) increases cAMP as expected in iWA, and CGP does not alter this effectNorepinephrine (NE) is also known to induce lipolysis, and liberated fatty acids are required to functionally activate UCP1 protein and to stimulate heat production. CREB phosphorylation at Ser133 is increased after Norepinephrine (NE) treatment and significantly attenuated with CGP co-treatment in mouse iWA.
RT-PCR
| Cell Line: | Subcutaneous preadipocytes Adipocytes. |
| Concentration: | 10 μM. |
| Incubation Time: | 6 hours. |
| Result: | AT2 activation suppressed Norepinephrine induced UCP1 in white adipocytes (iWA) |
