5’-Phosphoguanylyl-(3’,5’)-guanosine (pGpG) is an intermediate molecule produced by the pathway for enzymatic cyclic diguanylate (c-di-GMP) degradation. 5’-Phosphoguanylyl-(3’,5’)-guanosine can be used to detect the metabolism of nucleic acids[1][2].
5’-Phosphoguanylyl-(3’,5’)-guanosine reduces the rate of c-di-GMP degradation in cell lysates and inhibits the activity of EAL-dependent PDEs (PA2133, PvrR, and purified recombinant RocR) from P. aeruginosa[2].
[1]. Nesbitt NM, et, al. Modification of a bi-functional diguanylate cyclase-phosphodiesterase to efficiently produce cyclic diguanylate monophosphate. Biotechnol Rep (Amst). 2015 May 5;7:30-37. [2]. Cohen D, et, al. Oligoribonuclease is a central feature of cyclic diguanylate signaling in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2015 Sep 8;112(36):11359-64.
