4-Hydroxy-2,2,6,6-tetramethyl-piperidinooxy

TEMPOL is a piperidine nitroxide and spin label with superoxide dismutase (SOD) mimetic activity. It inhibits lipid peroxidation in rat liver microsomes with 50% inhibition of microsomal lipid peroxidation (IP₅₀) values of 117, 61, and 381 μM for peroxidation induced by iron plus NADPH, iron plus ascorbate, and t-butylhydroperoxide, respectively. TEMPOL (1 mM) inhibits production of superoxide anions by 92% via a xanthine-xanthine oxidase reaction in vitro. It reduces mean arterial pressure and heart rate in spontaneously hypertensive rats (ED₅₀s = 70 and 63 μmol/kg, respectively) when administered intravenously. TEMPOL is a cell-permeable spin label that has been used to quantify intracellular oxygen in various cell types by electron spin resonance (ESR) spectroscopy.

solid

Tempol, a water-soluble piperidine nitroxide derivative having nonspecific radical-scavenging and superoxide dismutase (SOD) activity, protects cultured aerobic, but not hypoxic, cells against radiation-induced killing. Protection does not depend on intracellular thiols and does not involve O2-depletion. Tempol reacts with peroxyl radicals and can also oxidize DNA-bound metal ions, thereby interfering with OH? generation.

A free radical scavenger

Spin label for EPR studies; phase transfer dehydration catalyst; antioxidant; inhibitor of olefin free radical polymerization.

In biochemical research, 4-hydroxy-TEMPO has been investigated as an agent for limiting reactive oxygen species.  It catalyzes the disproportionation of superoxide, facilitates hydrogen peroxide metabolism, and inhibits Fenton chemistry.4-Hydroxy-TEMPO, along with related nitroxides, are being studied for their potential antioxidant properties.On an industrial-scale 4-hydroxy-TEMPO is often present as a structural element in hindered amine light stabilizers, which are commonly used stabilizers in plastics, it is also used as a polymerisation inhibitor, particularly during the purification of styrene. 

Synthetic Communications, 19, p. 3509, 1989 DOI: 10.1080/00397918908052760

4-Hydroxy-TEMPO is a 4-substituted 2,2,6,6-tetramethylpiperidyl-1-oxy (TEMPO) derivative. It is a low-molecular weight compound and has been proposed as superoxide dismutase mimic.

Non flammable

Superoxide scavenger that displays neuroprotective, anti-inflammatory and analgesic effects.

Store at -20°C

[1] TATIANA LIPMAN  Philip L  Rinat Tabakman. Neuroprotective effects of the stable nitroxide compound Tempol on 1-methyl-4-phenylpyridinium ion-induced neurotoxicity in the Nerve Growth Factor-differentiated model of pheochromocytoma PC12 cells[J]. European journal of pharmacology, 2006, 549 1: Pages 50-57. DOI:10.1016/j.ejphar.2006.08.022
[2] GREGOR S GURON. Acute effects of the superoxide dismutase mimetic tempol on split kidney function in two-kidney one-clip hypertensive rats.[J]. Journal of Hypertension, 2006, 24 2: 387-394. DOI:10.1097/01.hjh.0000200511.02700.99
[3] AYELET M. SAMUNI . γ-Irradiation Damage to Liposomes Differing in Composition and Their Protection by Nitroxides[J]. Free Radical Biology and Medicine, 1997, 23 7: Pages 972-979. DOI:10.1016/s0891-5849(97)00123-8
[4] CATIA C F BERNARDY. Tempol, a Superoxide Dismutase Mimetic Agent, Inhibits Superoxide Anion-Induced Inflammatory Pain in Mice.[J]. BioMed Research International, 2017: 9584819. DOI:10.1155/2017/9584819
[5] MILES J. DE BLASIO . The superoxide dismutase mimetic tempol blunts diabetes-induced upregulation of NADPH oxidase and endoplasmic reticulum stress in a rat model of diabetic nephropathy[J]. European journal of pharmacology, 2017, 807: Pages 12-20. DOI:10.1016/j.ejphar.2017.04.026