iodoacetyll-LC-Biotin is a sulfhydryl-reactive biotinylation reagent that forms stable, irreversible thioether bonds when reacting with with sulfhydryls (-SH) group at alkaline pH. The hexylenediamine spacer arm gives this reagent the membrane-impermeability which enables intracellular labeling of biomolecules.
iodoacetyl-lc-biotin (n-iodoacetyl-n-biotinylhexylenediamine), a sulfhydryl-reactive biotinylation agent, is a water-insoluble reagent that requires the dissolution of suitable solvents, including dimethyl sulfoxide (dmso) and dimethylformamide (dmf), prior to the addition into aqueous reactions. iodoacetyl-lc-biotin consists of a bicyclic biotin rings structure, a 1,6-diaminohexane spacer group attached to the valeric acid side chain of biotin and an iodoacetyl group at the end of the spacer providing sulfhydryl reactivity. the iodoacetyl group at the end of iodoacetyl-lc-biotin is able to react with sulfhydryl groups on proteins and other molecules forming a nonreversible bond. the long spacer arm of iodoacetyl-lc-biotin enables the modified molecule to better bind to the avidin or streptavidin probes.bioconjugate techniques , 2nd ed. by greg t.hermanson (pierce biotechnology, thermo fisher scientific, rockford, il). academic press (an imprint of elsevier): london, amsterdam, burlington, san diego . 2008. isbn 978-0-12-370501-3.
