Anti-CDK4 (AB2) antibody produced in rabbit is suitable for western blotting at a concentration of 1 μg/ml.
Monoclonal Anti-Cdk4 antibody was used in:
- immunoprecipitation of human melanoma cell line.
- immunofluorescence of fibrosarcoma cells.
- western blot analysis
- immunofluorescence
- immunohistochemistry
Rabbit Anti-CDK4 (AB1) antibody can be used for western blot assays at 1μg/ml.
Cdk4 exists, in part, as a multi-protein complex with a D-type cyclin, proliferating cell nuclear antigen (PCNA) and a protein inhibitor, p21Cip1. Cdk4 associates separately with p16, particularly in cells lacking a functional retinoblastoma protein.
In association with cyclins, cyclin-dependent kinases (CDKs) forms active kinase complexes which is responsible for regulating cell cycle progression in eukaryotic cells. Within the complexes, the CDKs serves a catalytic protein kinase activity. This catalytic activity is regulated by two mechanisms: protein phosphorylation and association of regulatory subunits.
