Tau-412 human, (1N4R) recombinant, is one of the isoforms of Tau and lacks a projection domain region. The isoforms of tau are generated by alternate splicing. The N terminal region of tau is acidic and is separated from the C-terminal tubulin-binding region by a central proline-rich region. The isoforms differ in amino-terminal N1 and N2 regions as well as binding repeats at the tubulin region. Tau belongs to the family of neuronal microtubule-associated proteins.
Tau promotes assembly and stabilizes neuronal microtubules under normal physiological conditions. Under pathological conditions Tau can also undergo modifications such as hyperphosphorylation, which can result in the generation of aberrant aggregates such as found in neurofibrillary tangles in Alzheimer′s disease. Tau-412 human is purified without any acid treatment and is suitable for promoting microtubule assembly, and for hyperphosphorylation-induced self-assembly into filaments.
