Cholesteryl myristate

Cholesteryl myristate is a cholesterol ester. Levels of cholesteryl myristate are increased in the serum, liver, and aorta in rabbits fed a high-cholesterol diet and positively correlate with development of non-alcoholic fatty liver disease (NAFLD). Plasma levels of cholesteryl myristate also positively correlate with the presence of unstable atherosclerotic plaques in coronary artery disease. Cholesterol myristate has been used in the composition of lipid nanoparticles as drug carrier systems for drugs with low water solubility.

Cholesteryl Myristate is a natural steroid present in traditional Chinese medicine or used in tribal medicine.

ChEBI: A cholesterol ester obtained by the formal condensation of the hydroxy group of cholesterol with the carboxy group of myristic acid.

Human Endogenous Metabolite

Crystallise the myristate ester from Me2CO, EtOH/EtOAc or EtOH/Et2O or n-pentanol. Purify it also by column chromatography on silica gel and eluting with MeOH then evaporating to dryness. Recrystallise it and finally, dry it in vacuo over P2O5 and store it at -20o. [Labarière et al. Analyt Chem 30 1466 1958, Malanik & Malat Anal Chim Acta 76 464 1975, Beilstein 6 III 2638].

[1] EUNG JU KIM. Cholesterol-induced non-alcoholic fatty liver disease and atherosclerosis aggravated by systemic inflammation.[J]. PLoS ONE, 2014: e97841. DOI: 10.1371/journal.pone.0097841
[2] PETER J MEIKLE. Plasma lipidomic analysis of stable and unstable coronary artery disease.[J]. Arteriosclerosis, Thrombosis, and Vascular Biology, 2011, 31 11: 2723-2732. DOI: 10.1161/atvbaha.111.234096
[3] KARIN M. ROSENBLATT  Heike B. Evaluation of the drug loading capacity of different lipid nanoparticle dispersions by passive drug loading[J]. European Journal of Pharmaceutics and Biopharmaceutics, 2017, 117: Pages 49-59. DOI: 10.1016/j.ejpb.2017.03.010