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Product NameCTC
CAS90217-02-0
MFC16H14N5.Cl
MW311.77
EINECS
MOL File90217-02-0.mol

Chemical Properties

Melting point	225 ℃
storage temp.	2-8°C
solubility	Soluble in water
form	powder
color	Off-white
λmax	450nm (of formazan)
BRN	8954451
Biological Applications	NADPH-cytochrome P 450 reductase activity assays; detecting gamma-hydroxybutyric acid (GHB),metabolic activity,microorganisms; localizing glucose-6-phosphate dehydrogenase activity; monitoring respiratory activity; treating cancer
InChI	InChI=1S/C16H15N5.ClH/c1-13-3-7-15(8-4-13)19-12-18-20(11-17)21(19)16-9-5-14(2)6-10-16;/h3-10,12H,1-2H3;1H
InChIKey	BLCCXPMHHCWJHC-UHFFFAOYSA-N
SMILES	N1(C2=CC=C(C)C=C2)C=NN(C#N)[NH+]1C1=CC=C(C)C=C1.[Cl-]

Safety Information

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26
WGK Germany	3
F	8
HS Code	2933998090
Storage Class	11 - Combustible Solids
Hazard Classifications	Eye Irrit. 2 Skin Irrit. 2 STOT SE 3

MSDS

Provider	Language
SigmaAldrich	English

Usage And Synthesis

Description

5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride) is a redox-sensitive tetrazolium salt used primarily to detect metabolic activity in microorganisms. This membrane-permeable indicator is readily taken up by living cells and reduced by respiratory activity to produce a red fluorescent, water-insoluble formazan crystal (absorption maximum at 450 nm). While the oxidized salt is non-fluorescent, the reduced formazan can be evaluated using a standard rhodamine filter set (excitation: 510-560 nm; emission > 590 nm), although optimum excitation requires a blue 420 nm excitation filter. For flow cytometry, a 480 nm excitation laser combined with detection in the red region is suitable.

Uses

5-Cyano-2,3-di-p-tolyltetrazolium Chloride is a monotetrazolium redox dye that is used as a vital stain for actively respiring bacteria. Redox dye producing a fluorescent formazan (only in solid state) upon reduction; used as cellular indicator of respiratory activity in cells.

Uses

Redox dye producing a fluorescent formazan (only in solid state) upon reduction. It is used as cellular indicator of respiratory activity in cells, especiall for tracking P450 activity.

References

[1] G G RODRIGUEZ. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria.[J]. Applied and Environmental Microbiology, 1992, 58 6: 1801-1808. DOI: 10.1128/aem.58.6.1801-1808.1992
[2] G. CARUSO E. C M Mancuso. Combined fluorescent antibody assay and viability staining for the assessment of the physiological states of Escherichia coli in seawaters[J]. Journal of Applied Microbiology, 2003, 95 2: 225-233. DOI: 10.1046/j.1365-2672.2003.01967.x
[3] M E SIERACKI J N T L Cucci. Flow cytometric analysis of 5-cyano-2,3-ditolyl tetrazolium chloride activity of marine bacterioplankton in dilution cultures.[J]. Applied and Environmental Microbiology, 1999, 65 6: 2409-2417. DOI: 10.1128/aem.65.6.2409-2417.1999
[4] VISHVESH K BHUPATHIRAJU . Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria[J]. Journal of microbiological methods, 1999, 37 3: Pages 231-243. DOI: 10.1016/s0167-7012(99)00069-x

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