Carboxypeptidase B is an enzyme that specifically releases C-terminal lysine and arginine from substrates, and it was initially named "basic carboxypeptidase". It is distinguished from Anson's carboxypeptidase A and is also known as protaminase.
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 7.9, and the pI is 6.0.
Carboxypeptidase B has been used for the mass spectroscopy analysis ofanti-K11/K48 bispecific antibody.
Peptidyl-L-lysine (-L-arginine) hydrolase. Carboxypeptidase B is secreted as a zymogen (pro-carboxypeptidase B) from the pancreas.
Carboxypeptidase B is a Zn2+-metalloprotease, which catalyzes the hydrolysis of the basic amino acids L-Lys and L-Arg from the C-terminal position in polypeptides.
