石松鹼 性质
| 熔点 | 114-115°; mp (racemate) 130-131° |
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| 比旋光度 | D -24° (alc) |
| 沸点 | 125 °C(Press: 0.2 Torr) |
| 密度 | 1.18 g/cm3 |
| 储存条件 | Store at -20°C |
| 溶解度 | 溶于二甲基亚砜 |
| 酸度系数(pKa) | 9.36±0.40(Predicted) |
石松鹼 用途与合成方法
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Caspase-3
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5-Lipoxygenase
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Lycopodine (5.22-78.3 μg/mL; 12 hours) has 50% viability at 57.62±0.086 μg/mL and 51.46±1.43 μg/mL for PC3 and LnCaP, respectively.
Treated with Lycopodine (74-222 mM; 12 hours), the apoptotic index is with respect to the gradual increase in doses for the PC3 and LnCaP cells.
Lycopodine (74-222 mM; 12 hours) induces cell cycle arrest at G0/G1 phase in PC3 and LnCaP cells.
Lycopodine (0-200 µg/mL; 48 hours) shows cytotoxicity to HeLa cells in a dose and time dependent manner. However, Lycopodine shows minimal cytotoxic effects in normal peripheral blood mononuclear cells (PBMC) even at the highest dose (200 µg/mL).
Lycopodine (100, 200 µg/mL; 24 hours) increases level of Bax and decreases the mitochondrial cytochrome c. This is followed by an increase in expression of cytochrome c in cytosolic fraction. Lycopodine also cleaves the caspase-3 in the total cell lysate, while the expression of Bcl-2 is down regulated.
Cell Viability Assay
| Cell Line: | PC3 and LnCaP cells |
| Concentration: | 5.22-78.3 μg/mL |
| Incubation Time: | 12 hours |
| Result: | Reached 50% viability at 57.62±0.086 μg/mL and 51.46±1.43 μg/mL for PC3 and LnCaP, respectively. |
Apoptosis Analysis
| Cell Line: | PC3 and LnCaP cells |
| Concentration: | 74, 148, 222 mM |
| Incubation Time: | 12 hours |
| Result: | The apoptotic index was with respect to the gradual increase in doses. |
Cell Cycle Analysis
| Cell Line: | PC3 and LnCaP cells |
| Concentration: | 74, 148, 222 mM |
| Incubation Time: | 12 hours |
| Result: | Arrested growth of the cells at G0/G1 phase in the case of PC3 and LnCaP cells. |
Cell Cytotoxicity Assay
| Cell Line: | Hela cells and PBMC |
| Concentration: | 0-200 µg/mL |
| Incubation Time: | 48 hours |
| Result: | A linear increase of the cytotoxicity was along with the increase of time of treatment and also of the dose. |
Western Blot Analysis
| Cell Line: | HeLa cells |
| Concentration: | 100, 200 µg/mL |
| Incubation Time: | 24 hours |
| Result: | Increased level of Bax and decreased the mitochondrial cytochrome c. |
