Ultra sensitive fluorogenic substrate for ?-galactosidase, used in live cell and tissue (in vivo and in vitro) assays. This fluorescent substrate reagent could be used for monitoring kinetics of hydrolysis.
For detecting Glycosidases and could be used in ELISAs, FDG substrate has also been used for identifying lacZ-positive cells with fluorescence microscopy and flow cytometry
Fluorescein di(β-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting).
Fluorescein di (β-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by β-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using β-galactosidase (Lac Z) as a reporter gene.
