For detecting Glycosidases and could be used in ELISAs, FDG substrate has also been used for identifying lacZ-positive cells with fluorescence microscopy and flow cytometry
Fluorescein di(β-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting).
Ultra sensitive fluorogenic substrate for -galactosidase, used in live cell and tissue (in vivo and in vitro) assays. This fluorescent substrate reagent could be used for monitoring kinetics of hydrolysis.
Fluorescein di (β-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by β-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using β-galactosidase (Lac Z) as a reporter gene.
