Ac-QPKK(Ac)-AMC is a fluorogenic substrate for sirtuin 1 (SIRT1), SIRT2, and SIRT3.1 It is selectively deacylated by SIRT1-3 over SIRT6. Upon enzymatic cleavage by SIRT1, SIRT2, or SIRT3, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify SIRT1, SIRT2, and SIRT3 activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.
1.Galleano, I., Schiedel, M., Jung, M., et al.A continuous, fluorogenic sirtuin 2 deacylase assay: Substrate screening and inhibitor evaluationJ. Med. Chem.59(3)1021-1031(2017)
