There are three main production methods for nuclease P1: solid-state fermentation, deep liquid fermentation and immobilized liquid fermentation. Solid-state fermentation (solid-state fermentation, SSF) operation is simple, low cost, the use of bran and other crops as raw materials, but also be able to get a higher enzyme activity of the crude enzyme liquid. However, the composition of these agricultural products is very complex, thus, it is more difficult and cumbersome to obtain pure nuclease P1.
The deep liquid fermentation method utilizes modern fermentation technology, and through various sensors installed in the fermenter, the fermentation process can be better controlled, and the ratio of its raw materials is also clearer. Usually the main conditions of the liquid fermentation process: synthetic liquid medium, ventilation 1:0.4, fermentation temperature 30 , incubation time of 50 ~ 70 h. Different manufacturers of the process conditions based on their own use of strains and equipment have to be adjusted. Overall, liquid fermentation of mycelium and fermentation broth is easier to separate, is currently the main method of production of nuclease P1 crude enzyme liquid at home and abroad, widely used in nucleotide fermentation.
