SB 242235 (0-10 μM) dose-dependently inhibits the activation of MAPKAP K2 with an IC 50 of 1.0 μM in human chondrocytes stimulated with IL-1β.
SB 242235 inhibits intracellular p38 activity, MAPKAP K2 was then isolated from these cells and assayed using HSP27 as a substrate.
Western Blot Analysis
| Cell Line: | Human chondrocytes |
| Concentration: | 0 μM, 0.01 μM, 0.1 μM, 1 μM, 10 μM |
| Incubation Time: | < td class="col2"> 15 minutes
| Result: | Dose-dependently inhibited the activation of MAPKAP K2 with an IC 50 of 1.0 μM. |
