DSS Crosslinker is a membrane permeable, noncleavable crosslinker. The NHS ester groups are reactive toward primary amines.
Disuccinimidyl suberate (DSS) is a bifunctional cross-linking reagent which contains two N-hydroxysuccinimide esters that are reactive toward primary amines. DSS is membrane-permeable and is commonly used to cross-link proteins in intact cells.
Disuccinimidyl suberate is a homobifunctional amine-reactive crosslinker. It is usually coupled to molecules containing primary amines via amide bonds buffered at pH 7.5 (6.5-8.5). It incorporates an 8-atom spacer arm.
Suberic acid bis(
N-hydroxysuccinimide ester) has been:
- supplemented in pierce lysis buffer used for immunoprecipitation
- used to conjugate the oligonucleotide standards to gelatin
- used in cross-linking of ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain) oligomers
Suberic acid bis(N-hydroxysuccinimide ester) is a homobifunctional cross-linking reagent with amine reactivity. Typically coupled to molecules containing primary amine by amide bond buffered at pH 7.5 (6.5-8.5). Suberic acid bis(N-hydroxysuccinimide ester) incorporates an 8-atom linker.
