p62, also known as sequestosome-1 (SQSTM1), is a 62 kDa protein that acts as a signaling hub and autophagy substrate and adaptor. It is a multi-domain protein that includes a Phox1 and Bem1p (PB1) domain, a zinc finger, a tumor necrosis factor receptor-associated factor 6 (TRAF6) binding domain, a ubiquitin-associated (UBA) domain, LC3- and Keap1-interacting regions, as well as two nuclear localization and one nuclear export sequence. p62/SQSTM1 is constitutively expressed and is primarily localized in the cytoplasm, however, it is also expressed in the nucleus, autophagosomes, lysosomes, and inclusion bodies containing polyubiquitinated protein aggregates. It is overexpressed in a variety of human cancer cells as well as in the chronic liver diseases alcoholic and non-alcoholic steatohepatitis (NASH). p62/SQSTM1 binds to ERK1, RIP1, TRAF6, Raptor, PKC, LC3, and Keap1 to activate mammalian target of rapamycin complex 1 (mTORC1), NF-κB, and nuclear factor erythroid 2-related factor 2 (NRF2) signaling in response to oxidative and endoplasmic reticulum (ER) stress. It functions as a cargo receptor in selective autophagy to shuttle aggregated and damaged proteins and organelles to autophagosomes for clearance. Mutations in the UBA domain of the SQSTM1 gene are associated with Paget''s diseases. Cayman''s p62/SQSTM1 Polyclonal Antibody can be used for WB and ELISA applications. The antibody recognizes p62/SQSTM1 at ~60 kDa from human samples.
