MES monohydrate

한글명:MES monohydrate

카스 번호145224-94-8

상품명:MES monohydrate

CBNumberCB7485620

분자식C6H15NO5S

포뮬러 무게213.25

MOL 파일145224-94-8.mol

화학적 성질

녹는점	>300 °C(lit.)
밀도	10.66g/mL
벌크 밀도	570kg/m3
저장 조건	Sealed in dry,Room Temperature
용해도	H₂O: 0.5 M at 20 °C, 투명
물리적 상태	분말, 결정 또는 플레이크
색상	흰색에서 연한 노란색 및/또는 주황색
수소이온지수(pH)	2.5-4.0 (25℃, 0.5M in H2O)
산도 계수 (pKa)	6.1(at 25℃)
pH 범위	5.5 - 6.7
냄새	냄새 없는
생물학적 소스	synthetic
수용성	물에 용해됩니다.
최대 파장(λmax)	λ: 260 nm Amax: 0.025 λ: 280 nm Amax: 0.020
Merck	14,5902
BRN	141319
InChI	InChI=1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
InChIKey	MIIIXQJBDGSIKL-UHFFFAOYSA-N
SMILES	S(=O)(=O)(O)CCN1CCOCC1.O
CAS 데이터베이스	145224-94-8(CAS DataBase Reference)

위험 및 안전 성명

위험품 표기

위험 카페고리 넘버

36/37/38

안전지침서

26-36-37/39

WGK 독일

RTECS 번호

QE3479500

TSCA

Yes

HS 번호

29349990

NFPA 704:

	1
0		0

그림문자(GHS)

MES monohydrate 화학적 특성, 용도, 생산

개요
MES Monohydrate is a zwitterionic buffer that is not absorbed through cell membranes and is virtually transparent in UV light. MES is a buffering agent used in many biological and biochemical applications. It is also used as a running buffer for denaturing gel electrophoresis. The characteristics of low UV absorptivity, minimal reactivity, stable pH and solubility in water allow MES Monohydrate to be used as a Good's buffer.
화학적 성질
white crystalline powder
용도
MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. MES complexes with some organotin(IV) molecules that are being explored as antitumor agents.
MES free acid or MES free acid monohydrate can be mixed with MES sodium salt to acquire desired pH. Alternatively, MES free acid or MES free acid monohydrate can be titrated with sodium hydroxide to attain desired pH.
주요 응용
MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.
MES Monohydrate has been used:
To adjust the pH of growth medium to 5.5-7.0 in an in vitro cell wall stress assay.
As a buffering agent to stabilize enzymatic solutions.
As a component of PAGE running buffer.
일반 설명
buffer substance MES
주의 사항
Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unkown.

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