N-Acetylneuraminic Acid Aldolase

N-Acetylneuraminic Acid Aldolase 화학적 특성, 용도, 생산

• 용도

  Sialic acid aldolase can be used to synthesize unnatural sugars of C(6) to C(10) for the design of antagonists and inhibitors of glycoenzymes.

• 일반 설명

  Sialic acid aldolases, or N-acetylneuraminate lyases, catalyze the reversible aldol cleavage of N-acetylneuraminic acid to form pyruvate and N-acetyl-D-mannosamine. In nature, N-acetylneuraminate lyase mainly occurs in pathogens.

• Purification Methods

  Purify the aldolase by extraction with H2O, protamine precipitation, (NH4)2SO4 fractionation, Me2CO precipitation, acid treatment at pH 5.7 and precipitation at pH 4.5. The equilibrium constant for pyruvate + n-acetyl-D-mannosamine N-acetylneuraminidate at 37o is 0.64. The Km for N-acetylneuraminic acid is 3.9mM in phosphate at pH 7.2 and 37o. [Comb & Roseman Methods Enzymol 5 391 1962.] The enzyme from hog kidney (cortex) has been purified 1700-fold by extraction with H2O, protamine sulfate precipitation, (NH4)2SO4 fractionation, heating between 60-80o, a second (NH4)2SO4 fractionation and starch gel electrophoresis. The Km for N-acetylneuraminic acid is 1.5mM. [Brunetti et al. J Biol Chem 237 2447 1962.]

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