Purify it via the acetone cake, then Ca-phosphate gel filtration (unabsorbed), lyophilised and then fractionated through a DEAE-22 cellulose column. The Km for diacetyl is 40WM, and for NADH it is 100WM in phosphate buffer at pH 6.1. [Burgos & Martin Biochim Biophys Acta 268 261 1972, 289 13 1972.]
