モノクローナル抗Β-アクチン抗体, ペルオキシダーゼ標識 マウス宿主抗体

モノクローナル抗Β-アクチン抗体, ペルオキシダーゼ標識 マウス宿主抗体 化学特性,用途語,生産方法

• 使用

  Rabbit anti-actin antibody can be used for western blot assays. The antibody can also be used for immunohistochemistry (1:25, using human or animal tissue sections) and indirect immunofluorescence (1:40, using chicken fibroblasts) applications.
  Used to study actin structure and function and to probe binding sites of actin-binding proteins. Protein lysates generated from embryonic mouse limb tissue was analyzed by western blot using actin as the loading control. Actin was detected using rabbit anti-actin at 1:8000. Actin detected by a rabbit anti-actin antibody was used as a loading control for MCF7 cell lysates. Actin was detected at 42 kDa.

• 一般的な説明

  Actin/ actin β(ACTB) is encoded by the gene mapped to human chromosome 7p15-p12. In mammals, actin protein consists of six different isoforms encoded by separate genes. The four isoforms αskeletal-actin, αcardiac-actin, αsmooth-actin, and γ smooth-actin are expressed in skeletal, cardiac and smooth muscle. The other two isoforms, β cyto-actin and γ cyto-actin are ubiquitously expressed.

• Biochem/physiol Actions

  Actin is a cytoskeletal protein that regulates cell motility, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Mutations in the gene encoding this protein have been associated with Baraitser-Winter cerebrofrontofacial syndrome.

モノクローナル抗Β-アクチン抗体, ペルオキシダーゼ標識 マウス宿主抗体 生産企業

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