Use this premixed, double-concentrated formulation containing PwoSuperYield DNA Polymerase, a new optimized buffer system, and PCR grade nucleotides, if your application requires better fidelity than provided by (FastStart) Taq DNA Polymerase. The product can be applied for:
Accurate amplification of DNA critical applications
High-throughput PCR
High fidelity PCR
Site-directed mutagenesis
Cloning
Gene expression
一般的な説明
Pwo SuperYield DNA Polymerase is originally isolated from Pyrococcus woesei. The enzyme accepts modified nucleotides for efficient labeling of nucleic acids by PCR. PCR products are blunt-ended directly useable for blunt-end ligation. Using the magnesium-containing reaction buffer supplied, the final MgCl2 concentration is 1.5mM. The core component of the Pwo Master is the proofreading Pwo SuperYield DNA Polymerase which has both polymerase activity and a 3′–5′ exonuclease (proofreading) activity. Pwo SuperYield DNA Polymerase combines Pwo DNA Polymerase with an optimized buffer system. This buffer system enhances the enzymatic properties of the enzyme, resulting in higher yields of the amplification without changing the fidelity of DNA synthesis.
