ANTI-HUMAN IGG (GAMMA-CHAIN SPECIFIC)-R-PHYCOERYTHRIN

ANTI-HUMAN IGG (GAMMA-CHAIN SPECIFIC)-R-PHYCOERYTHRIN 価格

ANTI-HUMAN IGG (GAMMA-CHAIN SPECIFIC)-R-PHYCOERYTHRIN 化学特性,用途語,生産方法

• 使用

  Anti-Human IgG (γ-chain specific), F(ab′)² fragment-R-Phycoerythrin antibody is suitable for use in flow cytometry . The antibody can also be used for direct immunofluorescence (1:32).

• 一般的な説明

  Affinity isolated antigen specific antibody is obtained from goat anti-human IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the γ-chain of human IgG. Goat anti-human IgG is conjugated to Sigma R-Phycoerythrin (Product No. P8912) by a modification of the method of Kronick Unbound phycoerythrin is removed by gel filtration chromatography
  Specificity for the γ-chain of human IgG is determined by Ouchterlony Double Diffusion (ODD). The antibody preparation is specific for human IgG when tested against purified human IgA, IgG, IgM, Bence Jones κ and Bence Jones Lambda myeloma proteins, prior to conjugation
  Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum results in single arcs of precipitation.

• Biochem/physiol Actions

  Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising of one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallisable (fc), comprises the H chain constant region.

ANTI-HUMAN IGG (GAMMA-CHAIN SPECIFIC)-R-PHYCOERYTHRIN 生産企業

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