デオキシリボヌクレアーゼ

危険有害性情報のコード(GHS)

• 絵表示(GHS)

  

• 注意喚起語

  Danger

• 危険有害性情報

  H334：吸入するとアレルギー、喘息または、呼吸困難 を起こすおそれ

• 注意書き

  P261：粉じん/煙/ガス/ミスト/蒸気/スプレーの吸入を避ける こと。

  P284：呼吸用保護具を着用すること。

  P501：内容物/容器を．．．に廃棄すること。

デオキシリボヌクレアーゼ 価格

デオキシリボヌクレアーゼ 化学特性,用途語,生産方法

• 外観

  液体

• 用途

  DNAエンドヌクレアーゼ。

• 使用上の注意

  Reaction Bufferは添付されておりません。

• 解説

  デオキシリボヌクレアーゼ,DNA分子鎖中のホスホジエステル結合の加水分解反応を触媒する酵素の総称．DNA鎖の内部からDNAを分解し，5′-ホスホモノエステル・オリゴヌクレオチドを産生するものに，ウシの膵臓からのデオキシリボヌクレアーゼⅠ(EC 3.1.21.1)，連鎖状球菌のヌクレアーゼ，大腸菌エンドヌクレアーゼⅠ，酵母，アカパンカビおよび T2 感染菌デオキシリボヌクレアーゼなどがある．3′-ホスホモノエステル・オリゴヌクレオチドを産生するものとして，ウシの脾臓，胸腺からのデオキシリボヌクレアーゼⅡ(EC 3.1.22.1)がある．DNAの末端からDNAを分解し，ヌクレオシド5′-リン酸を産生する大腸菌エキソヌクレアーゼⅠ，Ⅱ，Ⅲ，Ⅳ，λファージ感染大腸菌K12からのエキソヌクレアーゼ，2本鎖DNAの1本鎖部分のみを分解するヌクレアーゼS1などがある．種々のDNA組換え実験や遺伝子の解析に用いられている．

• 使用

  Deoxyribonuclease I is used for the removal of DNA from protein samples. DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture.1 It has also been used along with trypsin for the preparation of single cell suspension from rat testes. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis

• 一般的な説明

  DNase I, or Deoxyribonuclease I, is an endonuclease isolated from bovine pancreas.
  
  • Our DNase I Digests double str and single stranded DNA into oligo and mononucleotides.
  • Bovine pancreatic DNase exists as four isozymes, having isoelectric points for A, B, C and D: 5.22, 4.96, 5.06 and 4.78.3. The predominant form is A, with smaller amounts of B and C, and only minor amount of D.
  • DNase I structure resembles the structure of to exonuclease III. It includes two central sheets. Each β sheet is composed of six β-strands. This complex of β sheets is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.^[1]

• 臨床応用

  DNAse is a human endonuclease, normally present insaliva, urine, pancreatic secretions, and blood. The enzymecatalyzes the hydrolysis of extracellular DNA into oligonucleotides.Aerosolized recombinant human deoxyribonucleaseI (rhDNAse), dornase alfa, Pulmozyme, has beenformulated into an inhalation agent for the treatment of pulmonarydisease in patients with CF.Among the clinical manifestations of CF are obstructionof the airways by viscous, dehydrated mucus. Pulmonaryfunction is diminished, and microbes can become entrappedin the viscid matrix. A cycle of pulmonary obstruction andinfection leads to progressive lung destruction and eventualdeath before the age of 30 for most CF patients. The immunesystem responds by sending in neutrophils, and theseaccumulate and eventually degenerate, releasing largeamounts of DNA. The high levels of extracellular DNA releasedand the mucous glycoproteins are responsible for thedegenerating lung function. The DNA-rich secretions alsobind to aminoglycoside antibiotics typically used to treat theinfections. In vitro studies showed that the viscosity of thesecretions could be reduced by application of DNAse I.
  Pulmozyme is approved for use in the treatment of CFpatients, in conjunction with standard therapies, to reducethe frequency of respiratory infections requiring parenteralantibiotics and to improve pulmonary function. The dose isdelivered at a level of 2.5 mg daily with a nebulizer.Pulmozyme is not a replacement for antibiotics, bronchodilators,and daily physical therapy.

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