As gas–solid and gas–liquid
chromatographic techniques are only suitable for the
analysis of volatile and thermostable compounds with
relatively low molecular mass, HPLC methods can also
separate nonvolatile, thermolabile, and large molecules.
The excellent separating power, high sensitivity, and
considerable diversity of solutes that could be separated
with HPLC contributed to its popularity.
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High-performance liquid chromatography (HPLC) has
been developed as a rapid and sensitive method for
the separation and quantitative determination of a wide
variety of compounds present in complex matrices in
very low concentration.