Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by:
Western blotting using the Anti-HA antibody
Silver staining (or similar protein stain)
In coimmunoprecipitation.
一般的な説明
Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal)
