エノラーゼ、酵母製

エノラーゼ、酵母製 価格

エノラーゼ、酵母製 化学特性,用途語,生産方法

• 外観

  タンパク質1～30mg/ml, りん酸カリウム, 0.05～0.1mol/l, 50%グリセリン溶液(pH7.0±0.5)

• 性質

  二量体，2 Mg2+含有．解糖系酵素．2-ホスホグリセリン酸→ホスホエノールピルビン酸+H2O

• 解説

  解糖系酵素の一つ．グリセリン酸2-リン酸から水を除去してエノール形ピルビン酸のリン酸エステルを生じる反応を触媒する酵素．高等動物，植物，酵母，細菌など解糖系をもつ生物界に存在する．

• 天然物の起源

  ニワトリ：1～434酵母：2～437

• 使用

  The enzyme from Sigma has been used as an antigen during ELISA. the study used human granulocyte proteins to identify and characterize autoantibodies against catalase and α-enolase in patients with primary sclerosing cholangitis. It has been used to study temperature- and denaturant-induced yeast enolase denaturation using fourier transform infrared spectroscopy. It has also been used along with other proteins to study gradient chromatofocusing-mass spectrometry; a new technique for protein analysis.

• 定義

  An enzyme active in glycolysis that catalyzes the conversion of 2-phosphoglyceric acid to the phosphorylated enol of pyruvic acid.

• 一般的な説明

  Enolase (2-phosphoglycerate hydratase) is a cytosolic enzyme. This cytosolic metalloenzyme is encoded by three homologous genes in mammals. Enolase the from baker′s yeast is a homodimer containing two bound Mg²⁺ ions. The peptide consists of 436 amino acids and contains a single cysteine residue and corresponds to a molecular weight of 93 kDa. Two of the active site components include His191 and Arg414. Enolase is seen in archaea, bacteria and eukaryotes.

エノラーゼ、酵母製 生産企業

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