レクチン バンデイラエアシンプリシフォリア(BANDEIRAEA SIMPLICIFOLIA(GRIFFONIA SIMPLICIFOLIA))由来

レクチン バンデイラエアシンプリシフォリア(BANDEIRAEA SIMPLICIFOLIA(GRIFFONIA SIMPLICIFOLIA))由来 化学特性,用途語,生産方法

• 使用

  General Western Blot Protocol:
  
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml
  
  
  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

• 一般的な説明

  Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has A and B subunits, which recognizes αGalNAc and αGal-end groups respectively. Lectins are extracted from the seeds of the legume shrub Griffonia simplicifolia, which are carbohydrate binding proteins.

• Biochem/physiol Actions

  BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

レクチン バンデイラエアシンプリシフォリア(BANDEIRAEA SIMPLICIFOLIA(GRIFFONIA SIMPLICIFOLIA))由来 生産企業

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