The β-Gal Staining Set is used for the histochemical staining of cells and tissue sections demonstrating β-galactosidase activity.
一般的な説明
The β-Gal Staining Set detects bacterial β-Gal activity for direct visualization in transfected cells. Forms a blue precipitate and simplifies the histochemical staining of cells and tissue sections for light microscopy. Upon transfecting cells with a β-Gal construct (encoded by the lacZ gene), cells are fixed, washed in PBS (phosphate buffered saline) and stained with freshly prepared staining solution. After staining, cells are washed again in PBS and evaluated by microscopy. When long-term storage is desired, PBS is replaced by mounting media (e.g., glycerol). For immunohistochemical staining of cells or tissue sections, incubate the specimen with an antibody conjugated to bacterial β-galactosidase, according to standard protocols. Subsequently, the specimen is processed for detection of β-Gal as described for transfected cells.
