Amplification of multiple DNA fragments for single nucleotide polymorphism (SNP) genotyping
Semi-quantitative PCR
3-plex PCR
一般的な説明
KAPA2G Fast Multiplex PCR Kits contain a second-generation (2G) enzyme derived through a process of directed evolution. KAPA2G FAST HotStart? DNA Polymerase is an antibody-mediated hot start formulation engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq DNA polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes, allowing for more uniform multiplexed PCR. KAPA2G Fast HotStart PCR Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance and does not require specialized PCR consumables or thermocyclers.
Biochem/physiol Actions
DNA fragments generated with KAPA2G Fast DNA Polymerase have the same characteristics as DNA fragments generated with wild-type Taq DNA polymerase, and may be used for sequencing, restriction enzyme digestion and cloning. Like wild-type Taq, KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated. PCR products generated with KAPA2G Fast are 3′-dA-tailed and may be cloned into TA cloning vectors.
