DPNI

DPNI 価格

DPNI 化学特性,用途語,生産方法

• 使用

  In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR.

• 一般的な説明

  Isoschizomers
  The enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.
  Methylation sensitivity
  The enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.
  Typical ligation and recutting assay
  Dpn I fragments obtained by complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10μl buffer that contains 66mM Tris-HCl, 5mM MgCl₂, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 × Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.

DPNI 生産企業

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