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Alkaline Phosphatase

CAS No.9001-78-9
Chemical Name:Alkaline Phosphatase
CBNumber:CB9690084
Molecular Formula:C21H36N8O6
Formula Weight:496.56054
MOL File:9001-78-9.mol

Alkaline Phosphatase Property

Density 1.12 g/mL at 20 °C
storage temp. -20°C
form suspension
color white
Specific Gravity 1
PH ~7.6
biological source mouse
Water Solubility It is soluble in water.
Specific Activity 30-90units/mg protein (modified Warburg-Christian, in glycine buffer)
Stability Stability Incompatible with strong oxidizing agents
EWG's Food Scores 1
EPA Substance Registry System Alkaline phosphatase (9001-78-9)
Absorption 7.6 at 278nm (10 mg AP/ml)
UNSPSC Code 41116100
NACRES NA.77

Safety

Hazard Codes :B,Xi
Risk Statements :36/37/38
Safety Statements :26-36-24/25-22-23
WGK Germany :3
F :3-10
TSCA :Yes
HS Code :35079090
Toxicity :An enzyme with an alkaline pH optimum that hydrolyzes phosphate monoesters. Elevation of its activity in the serum usually indicates obstructive jaundice, Paget’s disease (osteitis deformans), or bone carcinoma.

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal wordWarning
Hazard statements H317
Precautionary statements P280

Alkaline Phosphatase Price More Price(41)

Brand: Sigma-Aldrich(India)
Product number: SAE0111
Product name : Phosphatase, Alkaline from human placenta
Purity: ≥10?units/mg solid (in glycine buffer)
Packaging: 100UNITS
Price: ₹12887.1
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: SAE0111
Product name : Phosphatase, Alkaline from human placenta
Purity: ≥10?units/mg solid (in glycine buffer)
Packaging: 1000UNITS
Price: ₹53180.1
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: SAE0063
Product name : Alkaline Phosphatase Recombinant
Purity: solution (high-activity)
Packaging: 15MG
Price: ₹137229.3
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: P8361
Product name : Phosphatase, Alkaline bovine
Purity: recombinant, expressed in Pichia pastoris, ≥4000?units/mg protein
Packaging: 5000UNITS
Price: ₹45043.8
Updated: 2022/06/14
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Brand: Sigma-Aldrich(India)
Product number: P7923
Product name : Phosphatase, Alkaline from bovine intestinal mucosa
Purity: buffered aqueous glycerol solution, ≥4,000?DEA units/mg protein
Packaging: 2000UNITS
Price: ₹15462.3
Updated: 2022/06/14
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Alkaline Phosphatase Chemical Properties,Usage,Production

Chemical Properties solid
Uses Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions.
Uses Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes.
Uses Alkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids.
General Description Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase.
Biochem/physiol Actions Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).
Purification Methods The E.coli supernatant in sucrose (20%, 33mM) in Tris-HCl pH 8.0 is purified through a DEAE-cellulose column and recrystallised. To the column eluates in 0.125M NaCl is added MgCl2 (to 0.01M) and brought to 50% saturation in (NH4)2SO4 by adding the solid (0.20g/mL). The mixture is centrifuged to remove bubbles and is adjusted to pH 8.0 (with 2N NaOH). Saturated (NH4)2SO4 at pH 8.0 is added dropwise until the solution becomes faintly turbid (~61% saturation). It is set aside at ~25o for 1hour (turbidity will increase). The mixture is placed in an ice bath for several minutes when turbidity disappears and a clear solution is obtained. It is then placed in a large ice bath at 0o (~5L) and allowed to warm slowly to room temperature in a dark room whereby crystals are formed appearing as a silky sheen. The crystals are collected by centrifugation at 25o if necessary. The crystalline solutions are stable at ~25o for many months. They can be stored at 0o, but are unstable when frozen. Cysteine at 10-3M and thioglycolic acid at 10-4M are inhibitory. This is reversed on addition of Zn2+ ions. Many organic phosphates are good substrates for this phosphatase. [Molamy & Horecker Methods Enzymol 9 639 1966, Torriani et al. Methods Enzymol 12b 212 1968, Engstrom Biochim Biophys Acta 92 71 1964.] Alkaline phosphatase from rat osteosarcoma has been purified by Me2CO precipitation and chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxylapatite. [Nair et al. Arch Biochem Biophys 254 18 1987.] Phosphoproteins (various). These are purified by adsorbing onto an iminodiacetic acid substituted agarose column to which are bound ferric ions. This chelate complex acts as a selective immobilised metal affinity adsorbent for phosphoproteins. [Muszyfiska et al. Biochemistry 25 6850 1986.]