Biological Activity
UBCS039, the first synthetic SIRT6 activator with EC50 of 38 μM, induces time-dependent activation of autophagy in multiple human tumor cell lines.
in vitro
UBCS039 (75 μM, 48 or 72 hours) induces deacetylation of SIRT6-targeted histone H3 sites in human cancer cells. It leads to autophagosome accumulation in human cancer cells.UBCS039 induces autophagy via AMP-activated protein kinase (AMPK) signaling pathway activation.
Western Blot Analysis
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Cell Line:
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Human H1299 cells.
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Concentration:
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75 μM.
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Incubation Time:
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48 and 72 hours.
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Result:
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Induced deacetylation of SIRT6-targeted histone H3 sites in human H1299 cells.
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Cell Proliferation Assay
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Cell Line:
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Human H1299 cells.
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Concentration:
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100 μM.
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Incubation Time:
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48 and 72 hours.
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Result:
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Led to a strong decrease of cell proliferation in a dose-dependent manner when compared with control or DMSO-treated cells, starting from day 3 of growth (48 h after treatment) in both H1299 and HeLa cell lines.
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