Uses
Remove unincorporated precursors from DNA labeled by nick translation, end labeling, polymerization reactions, and other labeling techniques.
Use the highly purified radiolabeled DNA in Southern blotting and other downstream applications.
General Description
Ready-to-use disposable columns designed to quickly and efficiently remove unincorporated precursors from labeled DNA. Quick Spin columns are designed for use in low-speed, swinging-bucket centrifuges.Quick Spin Columns are produced with material shown to have high recovery of radiolabeled DNA (≥90%).
- All the tedious, time-consuming steps involved in preparing columns have already been performed. The columns are pre-packed, pre-swollen, and quality tested to ensure maximal retention of unincorporated nucleotides (≥99%).
ContentsPre-packed, pre-swollen, pre-spun columns.
Suspension of Sephadex G-50 in STE buffer (10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 100 mM NaCl).
