Description
Thioester analogs of glycerophospholipids, in combination with Ellman’s reagent, are convenient colorimetric substrates for the measurement of phospholipase (PL) activity. Diheptanoyl thio-
PC is a commonly-
used colorimetric substrate for all PLA
2s, with the exception of cPLA
2 and platelet-
activating factor acetylhydrolase (PAF-
AH). Thioester analogs of glycerophospholipids, in combination with Ellman’s reagent, are convenient colorimetric substrates for the measurement of phospholipase (PL) activity. Diheptanoyl thio-
PC is a commonly-
used colorimetric substrate for all PLA
2s, with the exception of cPLA
2 and platelet-
activating factor acetylhydrolase (PAF-
AH). Heptanoyl thio-
PC is an analog of diheptanoyl thio-
PC that contains an ether-
linked saturated C
16 moiety at the
sn-
1 position rather than a heptanoyl thiol ester. Porcine pancreatic and bee venom sPLA
2 enzymes exhibit 10-
13 fold less activity when assayed with heptanoyl thio-
PC compared to diheptanoyl thio-
PC. This decrease in activity has not been thoroughly investigated.
