Description
Thioester analogs of glycerophospholipids, in combination with Ellman’s reagent, are convenient colorimetric substrates for the measurement of phospholipase (PL) activity. Diheptanoyl thio-
PC is a commonly-
used colorimetric substrate for all PLA
2s, with the exception of cPLA
2 and platelet-
activating factor acetylhydrolase (PAF-
AH). Thioester analogs of glycerophospholipids, in combination with Ellman’s reagent, are convenient colorimetric substrates for the measurement of phospholipase (PL) activity. Diheptanoyl thio-
PC is a commonly-
used colorimetric substrate for all PLA
2s, with the exception of cPLA
2 and platelet-
activating factor acetylhydrolase (PAF-
AH). Heptanoyl thio-
PC is an analog of diheptanoyl thio-
PC that contains an ether-
linked saturated C
16 moiety at the
sn-
1 position rather than a heptanoyl thiol ester. Porcine pancreatic and bee venom sPLA
2 enzymes exhibit 10-
13 fold less activity when assayed with heptanoyl thio-
PC compared to diheptanoyl thio-
PC. This decrease in activity has not been thoroughly investigated.
References
[1] H S HENDRICKSON R H D E K Hendrickson. Chiral synthesis of a dithiolester analog of phosphatidylcholine as a substrate for the assay of phospholipase A2.[J]. Journal of Lipid Research, 1983, 24 11: 1532-1537.
[2] LAURE J. REYNOLDS Edward A D Lori L Hughes. Analysis of human synovial fluid phospholipase A2 on short chain phosphatidylcholine-mixed micelles: Development of a spectrophotometric assay suitable for a microtiterplate reader[J]. Analytical biochemistry, 1992, 204 1: Pages 190-197. DOI:
10.1016/0003-2697(92)90160-9[3] SUNDERLAND D, UZOIGWE G N. Personal Communication[J]. Communications in Africa, 1880–1939, 2018, 73 1: 0. DOI:
10.4324/9781351112277-85[4] ROBERTS M F. Phospholipases: structural and functional motifs for working at an interface[J]. FASEB Journal, 1996, 10 10: 1159-1172. DOI:
10.1096/fasebj.10.10.8751718
