Uses
PPHP is a substrate for the measurement of peroxidase enzymes. PPHP has been used to quantitate the peroxidase activity of COX-1 and COX-2.
Biochem/physiol Actions
Hydroperoxide compound used to assay peroxidase activity. Reduced to 5-phenyl-4E-pentenol (PPA) by plant and animal peroxidases in the presence of reducing substrates by a reaction that can be monitored spectrophotometrically.
References
[1] Weller, et al. Enzymatic reduction of 5-phenyl-4-pentenyl-hydroperoxide: Detection of peroxidases and identification of peroxidase reducing substrates. Arch. Biochem. Biophys. 243(2), 633-643 (1985).