Uses
Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PP
i + H
2O → 2P
i.
It plays an important role in protein, RNA, and DNA synthesis.
Definition
An inorganic pyrophos-
phatase.
General Description
Pyrophosphatase from
E coli (E-PPase) has a broader pH optimum and contains four divalent cations per subunit. It comprises 175 amino acids with additional aspartate residue in the active site cavity. Structurally E-PPase is a homohexamer with six identical 20 kDa subunits. Magnesium is a cofactor for E-PPase.
Biochem/physiol Actions
Pyrophosphatase from E coli (E-PPase) is an essential enzyme in yeast and bacteria The active site residues are crucial for binding to magnesium.
