Description
2-NBDG is a fluorescent derivative of glucose whose uptake is competitively inhibited by D-glucose , but not L-glucose or sucrose, in
E. coli. It has been used to monitor glucose uptake by bacteria and live mammalian cells and in tumor biopsies. 2-NBDG displays excitation/emission maxima of 475/550 nm, respectively.
Uses
2-NBDG is a fluorescent glucose uptake indicator, used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry.
References
[1] KAZUAKI YOSHIOKA. A novel fluorescent derivative of glucose applicable to the assessment of glucose uptake activity of Escherichia coli[J]. Biochimica et biophysica acta. General subjects, 1996, 1289 1: Pages 5-9. DOI:
10.1016/0304-4165(95)00153-0[2] K YAMADA. Measurement of glucose uptake and intracellular calcium concentration in single, living pancreatic beta-cells.[J]. The Journal of Biological Chemistry, 2000, 275 29: 22278-22283. DOI:
10.1074/jbc.m908048199[3] A. LOAIZA L B O Porras. Glutamate Triggers Rapid Glucose Transport Stimulation in Astrocytes as Evidenced by Real-Time Confocal Microscopy[J]. The Journal of Neuroscience, 2003, 5 1: 7337-7342. DOI:
10.1523/jneurosci.23-19-07337.2003
