ANTI-PHOSPHOTYROSINE-AGAROSE Chemical Properties,Usage,Production
Uses
Monoclonal Anti-Phosphotyrosine-Agarose antibody produced in mouse has been used in:
immunoprecipitation experiments for affinity-purification of phosphotyrosine proteins
phosphotyrosine pulldown assays
PI3-kinase assay
Uses
Monoclonal anti-phosphotyrosine antibody produced in mouse has been used in the detection of tyrosine phosphorylation in proteins by 2D western blotting and western blotting.
General Description
As determined by ELISA and competitive ELISA, the antibody reacts specifically with phosphorylated tyrosine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP.
Biochem/physiol Actions
Tyrosine residue phosphorylation plays a key role in cell growth and differentiation.
