Uses
Primuline has been used as a reagent in the preparation of lipids for chloroplast lipid membrane. It has been used as a dye in preparative thin-layer chromatography (TLC).
Preparation
p-Methylaniline (2 Moore) and sulfur (4.5 atomic ratio) in 200 ~ 280 ℃ heat, with hair smoke from the sulphonated sulfuric acid alkali primrose spirit.
Biological Activity
Primuline, also known as direct yellow 59 or primuline yellow, is considered to be a more sensitive and non-destructive dye for lipids. It is widely used as a fluorescent dye in preparative thin-layer chromatography (TLC). Primuline is suitable for vital staining. It is used as a fluorochrome in plant tissues. Primuline is also used to stain pollen grains and lignified cell walls. In addition, it also helps in segregating intact and broken starch grains. at pH 8, primuline acts as a fluorochrome for mast cells.
Properties and Applications
green light yellow. Soluble in water for light yellow, with a light blue fluorescence. The strong sulfuric acid for light yellow, a green fluorescent, after diluted orange yellow precipitation. The dye solution to join strong hydrochloric acid has golden precipitation; Add thick sodium hydroxide solution for orange brown precipitation. Used for the cellulose fibre, silk dyeing, leather and biological shading. Silk dyeing in acetic acid bath, dyeing copper and iron ion in colored light basic unchanged.
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Standard
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Acid Resistance
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Alkali Resistance
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Light Fastness
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Soaping
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Water
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Fading
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Stain
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Fading
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Stain
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ISO
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3
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4-5
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1
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2
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3
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AATCC
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4
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1-2
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2
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4
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