Description
Spermine NONOate (136587-13-8) is a nitric oxide donor and dissociates to the free amine and NO in a pH dependent manner.? ? The half-life of Spermine NONOate in 0.1 M phosphate buffer at pH 7.4 is 39 min at 37 °C, or 230 min at room temperature.1? ? Dissociation is essentially instantaneous at a pH of 5.0.
Chemical Properties
Off-White Solid
Uses
A reagent which releases Nitric Oxide into aqueous solutions at controlled rates for in vitro studies.
General Description
Complex of nitric oxide (NO) with spermine. Convenient reagent for preparing aqueous solutions of NO. Half-life (t
?) of NO release = 230 min (PBS, pH 7.4, 22°C).
Biological Activity
A stable complex of nitric oxide and spermine used to generate a controlled release of nitric oxide in solution (EC 50 = 6.2 μ M for relaxation of rabbit aorta, and t=39 min at 37℃, pH 7.4, aqueous solution).
Biochem/physiol Actions
Product does not compete with ATP.
References
1) Keefer et al. (1996), “NONOates” (1-substituted diazen-1-ium-1,2-diolates) as nitric oxide donors: Convenient nitric oxide dosage forms; Methods Enzymol., 268 281
