Description
Acrylodan is a sulfhydryl-reactive fluorescent probe for protein structural studies.
Uses
Acrylodan, reacted with thiols, is sensitive to the local environmental dipolarity and dynamics within the binding pocket surrounding Cys34[1][2].
References
[1] Sarah F Stuart, et al. A two-step mechanism for the folding of actin by the yeast cytosolic chaperonin. J Biol Chem. 2011 Jan 7;286(1):178-84. DOI:
10.1074/jbc.M110.166256[2] K Flora, et al. Unfolding of acrylodan-labeled human serum albumin probed by steady-state and time-resolved fluorescence methods. Biophys J. 1998 Aug;75(2):1084-96. DOI:
10.1016/S0006-3495(98)77598-8
