Description
DEPMPO (157230-67-6) is a spin trap capable of detecting oxygen, nitrogen, sulfur and carbon-centered free radicals both in vitro and in vivo. DEPMPO is useful for distinguishing between superoxide-dependent pathways of hydroxyl radical generation, and those that are superoxide independent.
Uses
DEPMPO is the most efficient spin trap for the in vitro detection of O-, N-, S- and C-centered free radicals both invitro and invivo. It has a longer life-time than DMPO and can distinguish between superoxide-dependent and independent mechanisms that lead to the hydroxyl radical. It is less lipophilic (Kp=0.16) than DIPPMPO.
References
[1] KE JIAN LIU . Evaluation of DEPMPO as a spin trapping agent in biological systems[J]. Free Radical Biology and Medicine, 1999, 26 5: Pages 714-721. DOI:
10.1016/s0891-5849(98)00251-2[2] HONGLIAN SHI . Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species[J]. Archives of biochemistry and biophysics, 2005, 437 1: Pages 59-68. DOI:
10.1016/j.abb.2005.02.028[3] MARCEL CULCASI . EPR spin trapping evaluation of ROS production in human fibroblasts exposed to cerium oxide nanoparticles: Evidence for NADPH oxidase and mitochondrial stimulation[J]. Chemico-Biological Interactions, 2012, 199 3: Pages 161-176. DOI:
10.1016/j.cbi.2012.08.007[4] FRANCESCO TAMPIERI*. Comment on “Water-Soluble Fluorescent Probe with Dual Mitochondria/Lysosome Targetability for Selective Superoxide Detection in Live Cells and in Zebrafish Embryos”[J]. ACS Sensors, 2019, 4 11: 3080-3083. DOI:
10.1021/acssensors.9b01358
