Description
(5,6)-FAM diacetate NHS ester is a cell-permeable amine-reactive label for biomolecules.
This molecule is non-fluorescent until the acetate groups are cleaved by intracellular esterases resulting in the formation of a highly fluorescent fluorophore with emission in the green spectrum range (absorption maximum at ~492 nm, emission maximum at ~517 nm). Due to this characteristic, this product is used for tracking cells and flow cytometric evaluations and studying intracellular esterase.
Chemical Properties
5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN is Off-white to light yellow powder
Uses
5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN is a Non-fluorescent cell permeant amine-reactive probe for long term tracing of cell.
Uses
A 5(6)-Carboxyfluorescein (C178215) derivative used as a flurorescent probe. 5(6)-Carboxyfluorescein 3’,6’-Diacetate N-Succinimidyl Ester is used to monitor lymphocyte proliferation by covalently labelling long-lived intracellular molecules with fluorescent dye.
Uses
Amine-reactive succinimidyl ester can potentially be used for long-term pH studies of live cells, producing a conjugate with the pH-sensitive properties of carboxyfluorescein.
General Description
5-(and-6)-Carboxyfluorescein diacetate
N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.
Biochem/physiol Actions
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.
